Fig. 4From: Knockdown of PSCA induces EMT and decreases metastatic potentials of the human prostate cancer DU145 cellsSuccessful construction of stable knockdown of PSCA DU145 cell lines. a The mRNA level of PSCA was analyzed by QRT-PCR among DU145, DU145 scramble and DU145 shRNA#1. QRT-PCR indicated that the silencing PSCA level was up to 90.5 %. b The mRNA level of PSCA was analyzed by RT-PCR. c The protein level of PSCA was analyzed by Western blot **P < 0.001, show DU145VS DU145 shRNA#1Back to article page