Table 3 Primers used for detection of mRNA Expression by qRT-PCR
Gene | Sequences | |
|---|---|---|
Forward | Reverse | |
Human experiments | ||
PSCA | CCTAACGCAAGTCTGACCATGTATG | TGCAGGCGGATCTGTGTCAATA |
GAPDH | CGCTGAGTACGTCGTGGAGTC | GCTGATGATCTTGAGGCTGTTGTC |
E-cadherin | TCACGCTGTGTCATCCAACGG | TCACGCTGTGTCATCCAACGG |
β-catenin | CAGGAAGGGATGGAAGGTCTC | TACCACCCACTTGGCAGACC |
Vimentin | ATCCAAGTTTGCTGACCTCTCTGA | GACTGCACCTGTCTCCGGTACTC |
Fibronectin | GAGACACCTGGAGCAAGAAGG | GTGAGGCTGCGGTTGGTAAAC |
Snail | CTTCTCCTCTACTTCAGTCTCTTCC | TGAGGTATTCCTTGTTGCAGTATTT |
Slug | AACAGAGCATTTGCAGACAGGTC | GCTACACAGCAGCCAGATTCC |
Twist | CACCATCCTCACACCTCTGCATT | GCTGATTGGCACGACCTCTTGA |