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Fig. 3 | Cancer Cell International

Fig. 3

From: MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7

Fig. 3

Smad7 is a novel target gene of miR-519d in cervical cancer. a Potential binding pattern of miR-519d to the 3′-UTR of Smad7 mRNA and the construct information of mutant Smad7 3′-UTR. b Firefly luciferase reporter assay through co-transfecting Smad7wt-Luc or Smad7mt-Luc with a Renilla luciferase control plasmid (pRL-TK), along with NC miRNA or miR-519d mimics. Shown were relative luciferase acitivities normalized to NC miRNA group (*P < 0.05). c Quantitative RT-PCR analysis of Smad7 mRNA in cervical cancer cells after transfection with NC miRNA or miR-519d mimics (*P < 0.05). d Western blot analysis revealed that the protein level of Smad7 was markedly reduced following miR-519d overexpression in HeLa and SiHa cells

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Cancer Cell International

ISSN: 1475-2867

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