Fig. 6
From: The voltage gated Ca2+-channel Cav3.2 and therapeutic responses in breast cancer
Effect of CACNA1H (Cav3.2) overexpression on expression of mRNA markers associated with tumour progression and drug-resistance. SKBR3 parental cells were co-transfected with α1Ha + pEGFP-N1 (EGFP Cav3.2) or pCDNA3.1 + pEGFP-N1 (EGFP MOCK) and sorted into EGFP enriched and depleted populations 24 h after transfection. Results are expressed as fold change normalised to EGFP MOCK. EGFP Cav3.2 cells showed an approximate greater than 2 106 fold increase in expression compared to EGFP MOCK cells (n = 3 ±SD). Overexpression of Cav3.2 did not increase levels of markers associated with EMT (vimentin, snail), basal markers (KR5, KR6A ) and markers associated with drug-resistance (CXCR4, FOXM1, HSP90AA1) (n = 3 ±SD)