Fig. 1

Weight loss protected against HFD-mediated early BBC onset. a Model of study design 1. At 8 weeks of age, C3(1)-Tag mice were randomly assigned to 10 % (N = 28) and 60 % (N = 59) diet groups. After 3 weeks on diet, at 11 weeks of age, N = 28 mice on 60 % were switched to 10 % diet. Mice were monitored for tumor onset by palpation 3 times a week. Three weeks after tumor onset, mice were sacrificed. b Body weight was measured weekly. 10 % vs. 60–10 % (^{^}P < 0.05); 10 % vs. 60 % (*P < 0.01); and 60 % vs. 60–10 % (^#P < 0.0001). c Mice were tumor free until first tumor palpated (P < 0.01, 10 % vs. 60 %; 60–10 % vs. 60 %)