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Fig. 3 | Cancer Cell International

Fig. 3

From: Hypoxia-induced modulation of PTEN activity and EMT phenotypes in lung cancers

Fig. 3

Effect of modulating phosphorylation sites in the PTEN C-terminuson hypoxia-induced EMT in H358 cells. a The indicated cells were treated with vehicle or Dox for 24 h before hypoxia stimulation. And then, the cells were cultured under normoxia or hypoxia for a further 24 h in the absence or presence of Dox. Western blotting analysis for total PTEN (top) and p-PTEN (bottom) was carried out. A blot is representative of three independent experiments (GFP, left; GFP-WildPTEN, middle; GFPPTEN4A, right). b Western blotting analysis for fibronectin and E-cadherin in the indicated cells cultured under normoxia or hypoxia for 48 h was carried out. A blot is representative of three independent experiments. c F/E ratio in the treated cells was compared with that in cells treated with vehicle in the absence of Dox. Asterisk indicates p < 0.05

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