Fig. 5

Pemetrexed (PMX) increased CD26 expression in vitro. a Based on flowcytometry analysis, cell surface CD26 expression on JMN cells increased 6-24 h after treatment with 10 μM of PMX. b Based on Western blot analysis, the expression of CD26 protein was rapidly induced in whole cell lysates by treatment with 10 μM of PMX at 1–24 h. GAPDH was used as an internal control. All experiments were performed in triplicate and a representative experiment is shown