Fig. 6
Analysis of mitochondrial apoptotic molecules in treated MDA-MB-231 cells. Untreated (Cont) or treated cells with ASPE at 50 µg/mL (C50) and 100 µg/mL (C100). a Comparison of change in expression of Bax and Bcl-2 genes is mentioned in diagrams as fold change (ratio of target/reference gene) in MDA-MB-231 cells. Each data point represents the mean (±SEM) of three independent experiments. b ROS measurement by Amplex Red™. Cells were treated with different concentrations of ASPE and incubated for 12 h. The difference between absorbance for treated and untreated cells samples was measured with a fluorescence-based microplate reader using excitation at 530 ± 12.5 nm and fluorescence detection at 580 ± 25 nm. The determined for a no-H2O2 control reaction, was subtracted from each value. The Graph demonstrates the percentage of H2O2 from Amplex Red assay based on mean absorbance. Each data point in all diagrams is representing the mean (±SEM) of three independent experiments. Asterisks indicate significant difference (P < 0.05)