Fig. 1From: Influence of TP53 and CDH1 genes in hepatocellular cancer spheroid formation and culture: a model system to understand cancer cell growth mechanicsSpheroid growth methods: spheroid growth was recorded at 24 h intervals. Formation was successful in all five cell lines with at least 7 of 8 wells developing structures within 24 h of incubation. Media changes were performed on every other day according to droplet volumeBack to article page