Fig. 2

Percent reduction of alamarBlue^® reagent: absorbance and emission (535/595 nm) of cell culture media was measured on days 1,3,5,7 and 10 after seeding. Higher percentage reduction indicates more metabolically active cells. Most cell lines show an overall decreasing trend in % reduction indicating the presence of fewer live cells within cultures over time. The slight increase in % reduction found among the wt (day 3–7), null (day 5–7) and SNU-387 (day 5–7) cell lines may be attributed to active remodeling in which necrotic cells are shed from the tumor surface by live cells