Fig. 7From: Influence of TP53 and CDH1 genes in hepatocellular cancer spheroid formation and culture: a model system to understand cancer cell growth mechanicsTP53 immunofluorescence: staining performed with D0-1 TP53 primary antibody and Alexa Fluor® 488 secondary antibody. The TP53-wt lines, C3A and HepG2 show intermediate levels of staining. Minimal to no fluorescence was observed in TP53-null Hep3B and TP53-mutant SNU-387. The TP53-double mutant, SNU-475 showed high nuclear staining. Axioskop imaging was set at an exposure of 500 ms using a ×63 oil objective for all imagesBack to article page