Fig. 1

Overexpression of miR-210 inhibits cell growth. a Proliferation assay of CRC cell lines 24, 48 and 72 h after transfection with pre-miR-210 and a control miRNA, respectively. Cells were trypsinized and counted (mean ± SEM; n = 3; *p < 0.05; **p < 0.01; student’s t test). b Measurement of cell cycle distribution was performed 48 h after transfection of pre-miR-210 and a control miRNA, respectively. Cells were stained with PI and the DNA content was measured by FACS analysis. Data represent the mean of three independent experiments. c Pre-miR-210 and control miRNA transfected CRC cells were seeded into 6-well-plates (500 cells/well) 72 h post transfection and cultured for 7 days. The colonies were subsequently stained with crystal violet and counted (left panel; mean ± SEM; n = 3; *p < 0.05; **p < 0.01; student’s t-test). A representative picture is shown (right panel)