Fig. 2

Overexpression of miR-210 induces apoptosis in CRC cell lines. a 72 h post transfection with pre-miR-210 and a control miRNA, respectively, the percentage of apoptotic cells was determined by PI staining and FACS analysis. Data indicate the percentage of cells showing a sub-G₁-DNA content (mean ± SEM; n = 3; **p < 0.01; student’s t-test). Representative flow cytometric histograms are shown in Additional file 2: Figure S2. b CRC cell lines were transfected as described in A. Lysates were generated 72 h thereafter. Cleavage of Caspase 3 was determined by immunoblot analysis. c CRC cell lines were transfected as described in A. Lysates were generated 48 h thereafter. d qRT-PCR analysis of Bim mRNA expression in pre-miR-210 transfected CRC cell lines compared to control miRNA transfected cells. CRC cell lines were transfected as described in A. Isolation of RNA was carried out 48 h thereafter. The level of Bim mRNA expression was measured by quantitative RT-PCR analysis and normalized to internal 18S rRNA expression (mean ± SEM; n = 3; **p < 0.01; student’s t-test). e CRC cell lines were transfected as described in A. Lysates were generated 48 h (HCT116, SW707) and 72 h (SW480) thereafter. pAKT expression levels were determined by immunoblot analysis. pAKT expression levels were densitometrically quantified using ImageJ Software and normalized to total AKT expression