Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen

Fig. 3

Downregulation of Bim and upregulation of Mcl-1 inhibit miR-210-mediated apoptosis. a CRC cell lines were transfected with pre-miR-210 and a control miRNA, respectively. Concomitantly, they were transfected either with two different Bim-specific siRNAs or a control siRNA. 72 h post transfection, the percentage of apoptotic cells was determined by PI staining and FACS analysis. Data indicate the percentage of cells showing a sub-G1-DNA content (mean ± SEM; n = 3; *p < 0.05; student’s t-test). b CRC cells were transfected as described in A. Lysates were generated 72 h thereafter. The successful knockdown of Bim was monitored by immunoblot analysis. c CRC cell lines were transduced with Mcl-1 AdV and a control AdV, respectively. 24 h thereafter, cells were transfected with pre-miR-210 or a control miRNA. The percentage of apoptotic cells was determined 72 h after transfection by PI staining and FACS analysis. Data indicate the percentage of cells showing a sub-G1-DNA content (mean ± SEM; n = 3; *p < 0.05; **p < 0.01; student’s t-test). d CRC cell lines were transduced with Mcl-1 AdV and a control AdV, respectively. Lysates were generated 72 h thereafter

Back to article page