Fig. 4
From: MicroRNA-210 induces apoptosis in colorectal cancer via induction of reactive oxygen
Overexpression of miR-210 induces ROS generation. a Measurement of ROS generation was performed 48 h after transfection of pre-miR-210 and a control miRNA, respectively (mean ± SEM; n = 3; *p < 0.05; **p < 0.01; student’s t-test). b CRC cell lines were transfected as described in A. Lysates were generated 48 h thereafter. ISCU expression was analyzed by immunoblot analysis. c CRC cell lines were transfected as described in A. Lysates were generated 48 h thereafter. Caspase 2 processing was analyzed by immunoblot analysis. d MCF-7 breast cancer cells were transfected as described in A. Lysates were generated 72 h thereafter. Caspase 2 processing was analyzed by immunoblot analysis