Fig. 5

Inhibition of ROS generation affects miR-210 induced cell death. CRC cells were transfected with pre-miR-210 and a control miRNA, respectively. 24 h post transfection, cells were treated with NAC. a Measurement of ROS generation was performed 24 h post NAC treatment (30 mM) (HCT116: n = 1; SW480, SW707: n = 3; mean ± SEM; ***p < 0.001; *p < 0.05; student’s t-test). b Lysates were generated 48 h post NAC treatment (10 mM). Processing of Caspase 2 was analyzed by immunoblot analysis. c Lysates were generated 48 h post NAC treatment (10 mM). Bim expression levels were determined by immunoblot analysis. d The percentage of apoptotic cells was determined 48 h post NAC treatment (30 mM) by PI staining and FACS analysis. Data indicate the percentage of cells showing a sub-G₁-DNA content (mean ± SEM; n = 3; *p < 0.05; **p < 0.01; student’s t-test). e Lysates were generated 48 h post NAC treatment (10 mM). Cleavage of Caspase 3 was determined by immunoblot analysis