Fig. 2

Phenotypic and functional characterization of HCC40-CL. a Phase-contrast microscopy images of HCC40-CL cells at passages 20 (Magnification: 100×, 200×). b Albumin and AFP levels of HCC40-CL cells were measured by flow cytometry. Percentages of albumin+ and AFP+ cells were indicated in the histograms. Dotted line: isotype control; Solid line: Albumin or AFP antibody. c Growth curve of HCC40-CL cells (passage 20) as determined by MTT. d Wound healing assay showing the migration ability of HCC40-CL cells. A wound was made by scraping the cell monolayer with a 20 μL pipette tip. Cell movement was photographed and observed under phase-contrast microscopy