Fig. 4

TP53 mutational analysis in original tumor and adjacent non-tumor liver tissue, HCC40-CL and HCC40-PDX. a IHC staining of p53 in adjacent non-tumor liver tissue and tumor specimen, and HCC40-PDX. Magnification: 400×. b Expression of p53 protein in Hep3B (p53 null), primary tumor specimen, adjacent non-tumor liver tissue, HCC40-CL and HCC40-PDX by western blot. β-actin served as loading control. c Sequencing analysis of TP53 gene revealed a point mutation at codon 104 of exon 5 (CAG → CCG) (Gln → Pro) in tumor specimen, HCC40-CL, and HCC40-PDX, but not in adjacent non-tumor liver tissue