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Fig. 5 | Cancer Cell International

Fig. 5

From: Zinc induces epithelial to mesenchymal transition in human lung cancer H460 cells via superoxide anion-dependent mechanism

Fig. 5

Zinc induces intracellular superoxide anion generation in H460 cells. ai The cells were incubated with specific ROS probes, namely, DHE, DCFH2-DA, or HPF at 4 °C for 30 min prior to the treatment with zinc (0–50 µM) for 0–3 h and the fluorescence intensity was analyzed by a microplate reader and flow cytometry. Mean intensity was normalized to untreated control cells and represented as relative ROS levels. Values are means of independent triplicate experiments ± SD. *p < 0.05 versus untreated control. jk The cells were incubated with DHE at 4 °C for 30 min prior to pre-treatment with 50 µM MnTBAP (superoxide anion inhibitor) for 1 h in the presence or absence of zinc (50 µM) for 3 h and the fluorescence intensity was analyzed by a microplate reader and flow cytometry. Mean intensity was normalized to untreated control cells and represented as relative ROS levels. Values are means of independent triplicate experiments ± SD. *p < 0.05 versus untreated control

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