Skip to main content
Fig. 7 | Cancer Cell International

Fig. 7

From: Zinc induces epithelial to mesenchymal transition in human lung cancer H460 cells via superoxide anion-dependent mechanism

Fig. 7

Zinc promotes EMT-related cancer aggressive behaviors of H460 cells through superoxide anion-dependent mechanism. a For cell migration, cells were incubated with 5 µM DMNQ (superoxide anion inducer) or 50 µM MnTBAP (superoxide anion inhibitor), in the presence or absence of zinc (50 µM) for 24 h before the assay. After 24 h of incubation, wound spaces were analyzed and represented as a relative migration level. b The relative cell migration was determined by comparing the relative change of those in untreated cells. cd For invasion, cells were incubated with 5 µM DMNQ or 50 µM MnTBAP in the presence or absence of zinc (50 µM) for 24 h and cells invasion was evaluated using a transwell coated with matrigel as described under “Methods” section. Value was represented as average number of invaded cells in each field relatively to control. e For tumorigenicity, cells were incubated with 5 µM DMNQ or 50 µM MnTBAP in the presence or absence of zinc (50 µM) for 24 h prior to grow in matrigel. After 10 days, colonies were visualized under microscope; scale bar 500 µm. f Value was represented as average diameter and number of colonies in each field relatively to control cells using image analyzer. Values of all experiments are means of independent triplicate experiments ± SD. *p < 0.05 versus untreated control. #p < 0.05 versus 50 µM zinc-treated cells

Back to article page