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Fig. 4 | Cancer Cell International

Fig. 4

From: miRNA-135a promotes hepatocellular carcinoma cell migration and invasion by targeting forkhead box O1

Fig. 4

miR-135a downregulated FOXO1 by directly targeting the FOXO1 3′ UTR. a Predicted miR-135a target sequences in 3′ UTR of FOXO1 (FOXO1-3′UTR) and mutant containing three mutated nucleotides (in green) in FOXO1-3′UTR (FOXO1-3′UTR-mut). b Luciferase assays of HepG2 cells transfected with pGL3 control, pGL3-FOXO1-3′UTR, or pGL3-FOXO1-3′UTR-mut reporter and 20 nM or 50 nM miR-135a mimic. c Western blot of FOXO1 expression after miR-135a overexpression or knockdown on HepG2. Error bars represent mean ± SD of three independent experiments. *p < 0.05

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