Fig. 1
Two-dimensional gel electrophoresis and MALDI-TOF mass spectra analysis reveal calreticulin as over expressed in breast cancer tissues. a A representative 2D gel electrophoresis map for the tumor tissue from a stage III sample after running on 17 cm pH 4–7 IPG strips. Spots 1 through 9 were excised and the protein identification was determined by MALDI-TOF analysis, in which spots 1–5 and spot 9 were over-expressed in the tumor tissue, while spots 6–8 were down-regulated. b MALDI-TOF mass spectrum of the tryptic peptides corresponding to calreticulin. c Expression of calreticulin in different intra ductal carcinoma (IDC) samples (T tumoral tissue, N normal adjacent tissue). d Graph represents the CRT expression fold change across different stages of infiltrating ductal breast carcinoma specimens