Fig. 2

Immunohistochemistry results and the comparison of the expression levels of calreticulin in various invasive states of breast cancer. a Immunohistochemical staining of calrecticulin expression in non-malignant lesions of human breast. (I) Negative control. (II) Positive staining in the glandular (white arrow) and stromal compartments (black arrow), ×10 magnification. (III) Positive staining in glandular (white arrow) and stromal compartments (black arrow), ×40 magnification. b Immunohistochemical staining of calreticulin expression in malignant lesions of human breast. (I) Negative control. (II) Positive staining in malignant glandular epithelium (black arrows) and stromal parts (white arrows) of the tumor, ×10 magnification. (III) Positive staining in glandular parts of breast tumor. (IV) Breast tumor with invasive pattern, ×10 magnification. (V) Breast tumor with invasive pattern, ×40 magnification. (VI) Calreticulin expression in stromal cells with spindle shapes (arrows). c (I) Differential expression of calreticulin in different stages of breast cancer. Calreticulin expression is presented based on the localization in three different tissue compartments: glandular structures, stroma and invasive areas. The expression of calreticulin is stratified according to the stage of the tumor (2, 3, 4) and the intensity of expression (low, moderate, high). (II) Differential expression of calreticulin in different grades of breast cancer. Calreticulin expression is presented based on the localization in three different tissue compartments: glandular structures, stroma and invasive areas. The expression of calreticulin is stratified according to the grade of the tumor (1, 2, 3) and the intensity of expression (low, moderate, high)