Fig. 3

The knockdown of CRT expression results in the inhibition of the migratory and invasive potential of MCF7 breast cancer cells. a Relative CRT expression analysis shows that more than 87 % decrease in expression was achieved in CRT-knockdown cells. b Western blot results confirmed the down regulation of calreticulin expression in CRT-knockdown MCF7 cells as compared to control groups. Beta actin was used as the house-keeping control. c MTT assay showed 90.7 % viability in CRT-siRNA transfected MCF7 cells as compared to the untreated group. d Migration assay results revealed a significant association between CRT expression and the migratory potential of MCF7 cells. The effect on migration was not proliferation-dependent. e Statistical analysis of the invasion assay results showed a significant decrease in the number of invading cells following CRT-knockdown of MCF7 cells