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Fig. 1 | Cancer Cell International

Fig. 1

From: PARG deficiency is neither synthetic lethal with BRCA1 nor PTEN deficiency

Fig. 1

The BRCA1-deficient MDA-MB-436 and HCC1937 cells but not the BRCA-proficient MDA-MB-231 and MCF10A cells are slightly sensitive to PARG depletion. a The MDA-MB-436 cell line shows increased sensitivity to PARP inhibition compared to the MDA-MB-231 cell line. Colony formation assays were performed with indicated concentrations of PARP inhibitor KU0058948. Mean values of triplicates (± SD) from one representative of 5 independent experiments are shown. b The BRCA1-proficient MDA-MB-231 cell line is not sensitive to PARG depletion. Left panels Clonogenic survival of MDA-MB-231 cells transfected with siCTL, siPARG, AllNeg or siPARG5. Results are from 6 (siCTL and siPARG) and 3 (AllNeg and siPARG5) independent experiments. Middle panels Percentage of viable cells relative to non-targeting siRNA transfected cells 72 h post-transfection, time point when cells are re-plated for clonogenic or short-term MTS assay. Results show mean values ± SD of 7 (siCTL and siPARG) and 5 (AllNeg and siPARG5) independent experiments. Right panels Cell viability measured by MTS assays 144 h post-transfection. Results show the percentage of viability relative to cells transfected with non-targeting siRNA from 3 independent experiments. Lower panels PARG depletion was verified by western blot at the time post-siRNA transfection indicated. #: non-specific band. c Clonogenic survival (left panels), cell counting at 72 h post-siRNA transfection (middle panels) and short-term MTS assay at 144 h post-siRNA transfection (right panels) were performed in MDA-MB-436 cell line as described in b. Number of experiments was 7 (siCTL and siPARG) and 3 (AllNeg and siPARG5) for clonogenic assays, 8 (siCTL and siPARG) and 4 (AllNeg and siPARG5) for cell counting at 72 h and 3 for MTS assay at 144 h post-siRNA transfection. Lower panels PARG depletion was verified by western blot at the time post-siRNA transfection indicated. d Clonogenic survival of MCF10A (left panel) and HCC1937 (right panel) cells transfected with siCTL and siPARG. Results are depicted as in a from 6 independent experiments. p: *** < 0.001; 0.001 < ** < 0.01; 0.01 < * < 0.05; 0.05 < ns, not significant

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