Skip to main content
Fig. 2 | Cancer Cell International

Fig. 2

From: PARG deficiency is neither synthetic lethal with BRCA1 nor PTEN deficiency

Fig. 2

The BRCA1-mutated UWB1.289 cell line is not more sensitive to PARG depletion than the BRCA1-reconstituted UWB1.289 + BRCA1 cell line. a Left panels Clonogenic survival of UWB1.289 cells transfected with siCTL, siPARG, AllNeg or siPARG5. Results are depicted as box plots showing distribution of data from 7 (siCTL and siPARG) and 4 (AllNeg and siPARG5) independent experiments. Middle panels Percentage of viable cells relative to non-targeting siRNA transfected cells 72 h post-siRNA transfection, time point when cells are re-plated for clonogenic or short-term MTS assay. Results show mean values ± SD of 11 (siCTL and siPARG) and 4 (AllNeg and siPARG5) independent experiments. Right panels Cell viability measured by MTS viability assays 144 h post-transfection. Results show the percentage of viability relative to cells transfected with non-targeting siRNA from 3 independent experiments. Lower panels PARG depletion was verified by western blot at the time post-siRNA transfection indicated. b Clonogenic survival (left panels), relative cell number at 72 h post-siRNA transfection (middle panels) and short-term MTS assay at 144 h post-siRNA transfection (right panels) were performed in UWB1.289 + BRCA1 cell line exactly as described in a. Results are depicted as box plots showing distribution of data from 7 (siCTL and siPARG) and 4 (AllNeg and siPARG5) for clonogenic assays. Number of experiments was 11 (siCTL and siPARG) and 4 (AllNeg and siPARG5) for cell counting at 72 h and 3 for MTS assay at 144 h post-siRNA transfection. Lower panels PARG depletion was verified by western blot at the time post-siRNA transfection indicated. c. Spontaneous PAR accumulation is a consequence of efficient PARG depletion in UWB1.289 (UWB) and UWB1.289 + BRCA1 (UWB + BRCA1) cells. PAR, BRCA1, PARG and actin levels were analysed by western blot using the indicated antibodies. BRCA1 specific signal is indicated by arrowheads. p: *** < 0.001; 0.001 < ** < 0.01; 0.01 < * < 0.05; 0.05 < ns, not significant

Back to article page