Fig. 5
From: PARG deficiency is neither synthetic lethal with BRCA1 nor PTEN deficiency
PARG deficiency is not synthetic lethal with PTEN deficiency. a The PTEN-mutated MDA-MB-468 cell line is not sensitive to PARG depletion. Clonogenic survival (left panel) of MDA-MB-468 cells after siCTL and siPARG transfection. Results are depicted as box plots showing distribution of data from 5 individual experiments. Relative cell number 72 h post-siRNA transfection (right panel) is counted from 5 individual experiments. PARG depletion was verified by western blot (middle panel). b Clonogenic survival (left panel) of MDA-MB-231 cells after single or combined siRNA-mediated depletion of BRCA1, PARG and PTEN. Results are depicted as box plots showing distribution of data from 5 independent experiments. Percentage of viable cells relative to siCTL-transfected cells 72 h post-transfection (right panel) is counted from 5 individual experiments. BRCA1, PARG and PTEN siRNA-mediated depletions compared to untransfected or siCTL transfected cells were verified by western blot (middle panel). The arrow points to BRCA1 signal above a non-specific band (#). p: 0.01 < * < 0.05; 0.05 < ns, not significant