Fig. 3
Top2β level dependent sensitivity to replication stress inducing drugs. Survival of U251 and NCH421k cells after exposure to increasing concentrations of selected drugs was determined by MTT assay as described in “Methods”. Drugs used were H2O2, CisPt, etoposide, CPT, MMS and TMZ. Green triangles U251 cells, blue diamond NCH421k control cells (siLuc), red squares NCH421k with Top2β down-regulation; average of three independent experiments ± SD is presented, asterisk indicates significance at p < 0.05. In the inset WB showing the efficiency of Top2β depletion in NCH421k cells