Fig. 5

Increased load of DNA damage in Top2β depleted cells. a DNA damage was induced using 200 μM methyl-methanesulphonate (MMS) or 50 μM cisplatin (CisPt) in control (Luc) and Top2β down-regulated NCH421k cells. Cells were stained for yH2AX (red) and counterstained with Toto3 (blue). Representative images are shown, scale bar 10 µm. b Number of foci per cell was quantified as described in “Methods” and is presented as the average of three experiments ± sd. c Chk1 activation after MMS and CisPt treatment, tubulin is a loading control. Below quantification of pChk1 normalized to tubulin