Fig. 3

KIF26B is a direct target of miR-20a-5p in OS cells. Expression levels of miR-20a-5p (a, b) and KIF26B mRNA (d, e) and protein (c) in miR-20a-5p mimic (5PM)-transfected SJSA-1 cells and miR-20a-5p antagomir (5PA)-transfected G-292 cells compared with the negative control (NC) as determined by qRT-PCR and Western blot analyses. f Layout of the luciferase reporter plasmid and the sequence of the WT and Mut UTR region of the KIF26B gene targeted by miR-20a-5p. g–i, The relative luciferase activity (fold change) of the reporter with WT or Mut KIF26B-UTR or with no UTR (Vec) was determined in the miR-20a-5p mimic (in SJSA-1)- or antagomir (in G292)- or Mock-transfected cells. Renilla luciferase activity of a co-transfected control plasmid was used as a transfection efficacy control. Representative results from three independent experiments are shown. Error bars represent the s.e.m. **p < 0.01 by Student’s t-test