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Fig. 3 | Cancer Cell International

Fig. 3

From: MiR-20a-5p represses multi-drug resistance in osteosarcoma by targeting the KIF26B gene

Fig. 3

KIF26B is a direct target of miR-20a-5p in OS cells. Expression levels of miR-20a-5p (a, b) and KIF26B mRNA (d, e) and protein (c) in miR-20a-5p mimic (5PM)-transfected SJSA-1 cells and miR-20a-5p antagomir (5PA)-transfected G-292 cells compared with the negative control (NC) as determined by qRT-PCR and Western blot analyses. f Layout of the luciferase reporter plasmid and the sequence of the WT and Mut UTR region of the KIF26B gene targeted by miR-20a-5p. gi, The relative luciferase activity (fold change) of the reporter with WT or Mut KIF26B-UTR or with no UTR (Vec) was determined in the miR-20a-5p mimic (in SJSA-1)- or antagomir (in G292)- or Mock-transfected cells. Renilla luciferase activity of a co-transfected control plasmid was used as a transfection efficacy control. Representative results from three independent experiments are shown. Error bars represent the s.e.m. **p < 0.01 by Student’s t-test

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