Fig. 7

The effect of miR-20a-5p on both the in vivo growth and Dox chemoresistance of SJSA-1- and G-292-derived xenografts in nude mice. a The experimental scheme. SJSA-1 or G-292 cells were subcutaneously injected at two sites on the back of each nude mouse. Two sites/mouse, 6 mice for SJSA-1, and 6 mice for G-292. From the tenth day after cell injection, all six SJSA-1-generated tumors on the left side of the backs of the nude mice were intratumorally injected with 2 nM miR-20a-5p agomir, while the right side of their backs was injected with 2 nM Mock. G-292-generated tumors on the left side of the backs of the nude mice were injected with 2 nM miR-20a-5p antagomir, while the right side of their backs was injected with 2 nM Mock. After cell injection, 3 mice from SJSA-1 and 3 from G-292 were intraperitoneally injected with Dox (45 µg/mouse) once every 2 days for 4 times. The remaining 6 mice (3 from SJSA-1 and 3 from G-292) received PBS as a mock treatment control. b Image of the representative mice with tumors. The mean ± SD of tumor weight of the tumors from the same treatment was calculated, plotted (*p < 0.05), and summarized in c–f. g Levels of KIF26B in each group were determined by immunostaining and summarized in the Table (Magnification: 200×)