Fig. 1
Expression analyses of key regulators of cell cycle and apoptosis in SAHA-treated uterine sarcoma cells. a Western blot analysis of ESS-1 and MES-SA cells treated for 8 h with 3 µM SAHA and/or 100 ng/ml TRAIL. Cell extracts were prepared, subjected to SDS-PAGE, and immunoblotted with antibodies against p21WAF1, p53, PUMA (23 kDa), and β-tubulin (as loading control). b Quantification of p21WAF1 and PUMA protein expression in relation to beta-tubulin expression