Fig. 5

Downregulation of autophagy in TP53-rescued and SAHA-treated ESS-1 cells. a Fluorescence staining of TP53-WT resubstituted, TP53-637C>T control-transfected, or non-transfected ESS-1 cells that were treated with 3 µM SAHA with a monoclonal antibody against the autophagy-specific marker LC3. Graphs depict the mean fluorescence intensity of LC3 specific staining, 12 h after the initiation of treatment. Untreated, SAHA (3 µM)-, SAHA and TRAIL-treated (100 nM), as well as untreated TP53-WT-transfected cells were measured as controls. b For immunoblotting analysis with antibodies against mTOR (289 kDa) and phospho-mTOR (p-mTOR), TP53-WT- or TP53-637C>T control-transfected ESS-1 cells were analyzed 6 and 12 h after treatment with or without SAHA. Untreated or rapamycin-supplied (2 µM) cells served as controls. β-tubulin was used as loading control