Fig. 7

Si-RNA mediated knockdown of p53 expression in MES-SA cells. Immunoblotting analysis was used to monitor the amount of silencing of p53 expression, and the resulting mTOR (289 kDa) and p-mTOR expression indicating autophagic induction 36 h after transiently transfecting MES-SA cells with si-RNA for p53 (50 and 100 nM) or non-targeted control si-RNA (100 nM). SAHA-treatment (3 µM) was started 12 h before analysis. Untreated, only SAHA-treated, or rapamycin-supplied cells served as additional controls. β-tubulin was used as loading control