Fig. 8

Monitoring of TP53-rescued tumor cell lines for apoptosis and autophagy induction. a A TP53-proficient (HeLa) and several TP53-mutant and SAHA-responsive tumor cell lines (PANC-1, Jurkat, HL-60, and U937 cells) were transfected either with TP53-WT or with TP53-637C>T, respectively. After 24 h of 3 µM SAHA treatment, MDC and PI fluorescence was quantified in order to monitor resulting changes in autophagy and cytotoxicity. b To assess apoptotic activity in TP53-WT reconstituted tumor cell lines as described in a, caspase-3 and -7 activation (Caspase-Glo 3/7 Assay) was measured upon 3 µM SAHA treatment for 24 h and compared to untreated, or only SAHA-treated cells. c Additionally, the amount of LDH release was determined from cells used for the experiment in b. Statistically significant differences compared to the SAHA-treated control are indicated in a–c