Fig. 2
From: Robo3A and Robo3B expression is regulated via alternative promoters and mRNA stability
Robo expression in RASF as well as in melanoma cell lines. a mRNA levels of the two Robo3 isoforms in RASF in early and late passages using specific primers for both transcription variants. b Western blot analysis using the designed Robo3B-specific antibody (I.) showing the 150 kDa Robo3B protein (β-actin as reference, 42 kDa) and densitometric analysis normalized by β-actin (II.). c Robo3B mRNA expression relative to Robo3A in melanoma cell lines as well as in neuroglioma cell line (C3H4). d Protein expression of the tumor cell lines analyzed by the Robo3B-specific antibody (I.) and densitometric analysis normalized by β-actin (II.)