Fig. 4
From: Robo3A and Robo3B expression is regulated via alternative promoters and mRNA stability
Regulation of the two Robo3 isoforms. a–c Mel Ei were treated with several inhibitors for 24 h. The amount of Robo3A (A) respectively Robo3B (B) mRNA expression was quantified by real-time (RT)-PCR and showed varied expression pattern especially when comparing the ratio of Robo3B relative to Robo3A (C). d, e Reporter gene assays using specific Robo3A or the Robo3B promoter plasmid. Luciferase activity was measured in melanoma cell lines as well as in neuroglioma cell line (C3H4). d A significantly increased promotor activity of the Robo3B promotor compared to the Robo3A promotor was measured in all metastasis cell lines of melanoma (Mel Im, A375, HTZ19d) except Hmb2. e No significant differences were found in primary tumor of melanoma except Mel Juso. Also no significant differences in the neuroglioma cell line