Fig. 1

Hypoxia upregulates ANRIL expression in osteosarcoma cells. a Expression level of ANRIL by qRT-PCR in 15 osteosarcoma tissues and paired non-tumor tissues. GAPDH was used as a loading control. b ANRIL expression under both normoxic and hypoxic conditions were analyzed by real-time PCR. c ANRIL promoter activity under both normoxic and hypoxic conditions were analyzed by luciferase assay (**, P < 0.01, n = 3). β-actin was used as the internal control (**, P < 0.01, n = 3)