Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: RETRACTED ARTICLE: Long noncoding RNA ANRIL is activated by hypoxia-inducible factor-1α and promotes osteosarcoma cell invasion and suppresses cell apoptosis upon hypoxia

Fig. 4

Effects of ANRIL silencing on the viability and apoptosis of hypoxic osteosarcoma cells. a Knockdown efficiency of ANRIL siRNA was detected by real-time PCR. β-actin was used as the internal control (**, P < 0.01, n = 3). b Cell viability of ANRIL-silenced and negative control U2OS cells cultured under hypoxic conditions was detected by CCK-8 assay. Data are presented as a percentage of the control group (**, P < 0.01, n = 3). c Apoptosis of ANRIL-silenced U2OS cells and negative control cells under hypoxia conditions was measured by flow cytometry (**, P < 0.01, n = 3). d Bax and Bcl-2 protein levels were analyzed by Western blotting after ANRIL knockdown. β-actin was used as the internal control. (**, P < 0.01, n = 3). Data represent the mean ± S.D

Back to article page

Cancer Cell International

ISSN: 1475-2867

Contact us