Fig. 4

BMP2 sensitize CRC cells to 5-fluorouracil. a Illustration of the DNA damage response pathway based on microarray data with matched entities highlighted. b Representative fluorescence images of BMP2 and LV control HCT116 cells [±different concentration (1.5–100 μM) 5-fluorouracil]. Cells were stained with acridine orange/ethidium bromide to detect apoptotic (cells with green condensed chromatin) and necrotic cells (red) c. Representative clonogenic assay showing reduced clonogenicity of BMP2 HCT116 compared to LV control HCT116 cells (±1.5 μM 5-fluorouracil). Plates were stained with Diff-Quik stain set on day 10