Fig. 2

DBCCR1-003 regulates the expression of DBCCR1 via DNA hypermethylation. a, b The mRNA expression level of DBCCR1 and DBCCR1-003 in Lenti-DBCCR1-003 cells(L-D3) and T24 cells treated by DAC(DAC) compared to the mock and Lenti-vector cells (L-C) were tested by real-time PCR. c DBCCR1 protein expression in Lenti-DBCCR1-003 cells and T24 cells treated by DAC compared to the mock and Lenti-vector cells. The intensity of protein bands are quantified. 1 MOCK; 2 L-C; 3 L-D3; 4 DAC. d, e MSP analyses of DBCCR1 gene promoter in BC tumor and the matched adjacent tissues of BC. f MSP analyses of DBCCR1 gene promoter in Lenti-DBCCR1-003 cells and T24 cells treated by DAC compared to the mock and Lenti-vector cells. Results represent the mean + SD from three independent experiments. Asterisk indicate significant difference at p < 0.05