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Fig. 4 | Cancer Cell International

Fig. 4

From: Long non-coding RNA DBCCR1-003 regulate the expression of DBCCR1 via DNMT1 in bladder cancer

Fig. 4

DBCCR1-003 may bind to DNMT1 and prevent DNMT1-mediated the methylation of DBCCR1. a, b The mRNA and protein expression level of DNMT1 in Lenti-DBCCR1-003 cells (L-D3) and T24 cells treated by DAC (DAC) compared to the mock and Lenti-vector cells (L-C) were tested by real-time PCR and Western Blot, respectively. c RT-PCR analysis of DBCCR1-003 in RIP with anti-DNMT1. The enrichment of RNA was measured relative to the input levels using the 2CT method. Relative RNA binding levels of DBCCR1-003 and DNMT1 in Lenti-DBCCR1-003 cells and T24 cells treated by DAC compared to the mock and Lenti-vector cells. Results represent the mean + SD from three independent experiments. Asterisk indicate significant difference at p < 0.05

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