Fig. 2
From: Alpha-synuclein contributes to malignant progression of human meningioma via the Akt/mTOR pathway
Knockdown of α-synuclein (α-Syn) reduces cell proliferation and colony formation and increases apoptosis in meningioma cells. a Measurement of α-Syn mRNA and protein levels in IOMM-Lee meningioma cells transfected with control or α-Syn shRNA. b BrdU incorporation assay was performed to determine cell proliferation in IOMM-Lee cells transfected with control or α-Syn shRNA. c Colony formation assay. IOMM-Lee cells transfected with control or α-Syn shRNA were allowed to form colonies for 10 days. Top representative dishes. Bottom quantification of colony formation. d Apoptosis was quantified by flow cytometry. Top representative dot plots of cells stained with Annexin-V and PI. Bottom quantitative data from three independent experiments. *P < 0.05 versus cells transfected with control shRNA