Fig. 4

Integrin β3 is involved in the inhibition effect of kallistatin on proliferation of NCI-H446 cells. a Proliferation of NCI-H446 cells was inhibited in presence of kallistatin. Proliferating NCI-H446 cells were labeled with EdU (red). Cell nuclei were stained with Hoechst 33,342 (blue). b The percentage of EdU-positive NCI-H446 cells was quantified. Kallistatin inhibited the proliferation of NCI-H446 cells in a dose-dependent manner. Data were presented as mean ± SD (N = 6). *P < 0.05. c Down-regulation of integrin β3 by siRNA attenuated the proliferation inhibition of NCI-H446 cells at 48 h post kallistatin treatment as measured by EdU assay. d The percentage of EdU-positive NCI-H446 cells was quantified. Data were presented as mean ± SD (N = 6). N.C siRNA was used as negative control. *P < 0.05, ^#P < 0.05, ^# comparison of the differences between column 1 and 2 and between column 3 and 4. e Blockage of integrin β3 with antibody (anti-integrin β3) attenuated the viability inhibition of NCI-H446 cells at 48 h post kallistatin treatment as measured by EdU assay. f The percentage of EdU-positive NCI-H446 cells was quantified. Data were presented as mean ± SD (N = 6). Mouse IgG was used as negative control. *P < 0.05, ^#P < 0.05, ^# comparison of the differences between column 1 and 2 and between column 3 and 4