Fig. 5
Reduction effects of kallistatin on NCI-H446 cells migration can be diminished by inhibition of integrin β3. A Optical microscopy imaging of the cells in experimental transwells. The cells have migrated from upper chamber to underside of the membrane between the two chambers after treatment with kallistatin. a saline; b 12.5 μg/ml kallistatin; c 50 μg/ml kallistatin; d 200 μg/ml kallistatin. B Statistic analysis for the migrated cell numbers. C Either down-regulation of integrin β3 by siRNA or blockage of integrin β3 with antibody (anti-integrin β3) attenuated the reduction effects of kallistatin on NCI-H446 cell migration. a Transfected with negative control siRNA and treated with saline; b transfected with negative control siRNA and treated with 200 μg/ml kallistatin; c transfected with integrin β3 siRNA and treated with saline; d transfected with integrin β3 siRNA and treated with 200 μg/ml kallistatin; e treated with mouse IgG and saline; f treated with mouse IgG and 200 μg/ml kallistatin; g treated with integrin β3 antibody and saline; h treated with integrin β3 antibody and 200 μg/ml kallistatin. D Statistic analysis for the migrated cell numbers. Data were presented as mean ± SD (N = 6). *P < 0.05.#P < 0.05,# comparison of the differences between column 1 and 2 and between column 3 and 4, and the differences between column 5 and 6 and between 7 and 8