Fig. 6

Kallistatin inhibits phosphorylation along the integrin signaling pathway. a 200 μg total cellular protein of each sample was used for immunoblotting analysis. Phosphorylation of integrin β3, FAK and Src was inhibited in a time-dependent manner by treatment of kallistatin, while each of their total protein levels showed undetectable change. (N = 3). The protein levels were normalized with β-actin. b–d The relative levels of phosphorylated integrin β3, phosphorylated FAK and phosphorylated Src proteins were calculated and plotted. The data are expressed as mean ± SD from three independent experiments (N = 3). *P < 0.05