Fig. 6From: Autophagy induced by a sulphamoylated estrone analogue contributes to its cytotoxic effect on breast cancer cellsTransmission electron micrographs of MDA-MB-231 cells. MDA-MB-231 cells treated with a DMSO and b 3MA showed an intact nuclear membrane and well-defined cell membranes with minimal cell protrusions. c Actinomycin D-exposed cells showed membrane blebbing and apoptotic body formation. d Tamoxifen-treated cells showed more vesicle formation when compared to cells treated with e tamoxifen with 3MA. f ESE-15-ol-treated cells showed hyper condensed chromatin with increased vesicles and apoptotic body formation. g Cells exposed to ESE-15-ol with 3MA showed fewer vesicles with an intact nuclear membrane (Scale bar 5 μM) (Arrow colour keys: white = intact nuclear membrane; red = apoptotic bodies; yellow = vesicles)Back to article page