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Table 2 Semi-quantitative evaluation of immunocytochemical reaction with anti-PARP-1 and anti-caspase 3 antibodies in Me-45 cells after exposition to betulin derivatives, after 24 h

From: Anticancer properties of ester derivatives of betulin in human metastatic melanoma cells (Me-45)

Compound Me-45
PARP-1 12.5 µM PARP-1 6 µM Casp 3 12.5 µM Casp 3 6 µM
Control cells 5%, +/++ <5%,
Betulin‐l ‐Lys‐NH2 95%, ++ 50%, +/++ 0% 0%
Betulin‐l ‐Orn‐NH2 100%, ++ 98%, +/++ <5%, <5%,
Betulin‐l ‐Dab‐NH2 100%, +++ 100%, +++ 0% 0%
Betulin‐l ‐Dap‐NH2 100%, +++ 98%, +/++ <5%, <5%,
Betulin‐l ‐Ala‐NH2 100%, ++/+++ 90%, ++ <5%, 0%
Betulin 100%, ++/+++ 95%, ++/+++ 10%,+ <5%,
Betulinic acid 100%, ++/+++ 100%, ++/+++ 5-10%, +/++ <5%,