Table 2 Semi-quantitative evaluation of immunocytochemical reaction with anti-PARP-1 and anti-caspase 3 antibodies in Me-45 cells after exposition to betulin derivatives, after 24 h
From: Anticancer properties of ester derivatives of betulin in human metastatic melanoma cells (Me-45)
Compound | Me-45 | |||
|---|---|---|---|---|
PARP-1 12.5 µM | PARP-1 6 µM | Casp 3 12.5 µM | Casp 3 6 µM | |
Control cells | 5%, +/++ | – | <5%,∓ | – |
Betulin‐l ‐Lys‐NH2 | 95%, ++ | 50%, +/++ | 0% | 0% |
Betulin‐l ‐Orn‐NH2 | 100%, ++ | 98%, +/++ | <5%, ∓ | <5%, ∓ |
Betulin‐l ‐Dab‐NH2 | 100%, +++ | 100%, +++ | 0% | 0% |
Betulin‐l ‐Dap‐NH2 | 100%, +++ | 98%, +/++ | <5%, ∓ | <5%,∓ |
Betulin‐l ‐Ala‐NH2 | 100%, ++/+++ | 90%, ++ | <5%, ∓ | 0% |
Betulin | 100%, ++/+++ | 95%, ++/+++ | 10%,+ | <5%,∓ |
Betulinic acid | 100%, ++/+++ | 100%, ++/+++ | 5-10%, +/++ | <5%, ∓ |