Table 1 Primers used with respective amplicon size and annealing temperatures
From: Alginate-embedded HuH-7 cells increase MMP-9 and reduce OCLN expression in vitro
Gene | Primer sequence | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|
MMP-9 | f: 5′-GCCCAGCCCACCTCCACTCCTC-3′ | 173 | 60 |
r: 5′-TGGGCTACGTGACCTATGACAT-3′ | |||
OCLN | f: 5′- ACAAGCGGTTTTATCCAGAGTC-3′ | 89 | 60 |
r: 5′-GTCATCCACAGGCGAAGTTAAT-3′ | |||
p65 | f: 5′-GGGACTACGACCTGAATGCT-3′ | 100 | 62 |
r: 5′-ATGGGATGAGAAAGGACACG-3′ | |||
I-CAM 1 | f: 5′-ATGCCCAGACATCTGTGTCC-3′ | 111 | 60 |
r: 5′-GGGGTCTCATGCCCAACAA-3′ | |||
VEFG | f: 5′-AGGGCAGAATCATCACGAAGT-3′ | 75 | 60 |
r: 5′-AGGGTCTCGATTGGATGGCA-3′ | |||
GAPDH | f: 5′-CCCATCACCATCTTCCAGG- | 149 | 62 |
r: 5′-GAGATGATGACCCTTTTGGC-3′ |