Fig. 1

Casticin inhibits the proliferation and viability of NOZ and SGC996 cells. a The chemical structure of casticin. b, c NOZ, SGC996 and 293T cells were treated with various concentrations of casticin (0, 0.1, 0.5, 1, 4, 7 µM) for 24, 48 or 72 h. Cell viability was assessed using the CCK-8 assay. d NOZ and SGC cells were exposed to 1 µM casticin for 24 h, 48 or 72 h. f, g Casticin suppressed colony formation of NOZ and SGC996 cells. Cells were exposed to casticin (0, 1, 4, 7 µM) and were allowed to form colonies for 14 days. All data are presented as the means ± standard deviations, and each experiment was repeated 3 times. Significant differences compared with the control are indicated by *p < 0.05, **p < 0.01, and ***p < 0.001